(230) Chronic Unpredictable Stress Impairs Corpus Cavernosum Reactivity in Mice

Abstract Introduction Stress is associated with erectile dysfunction (ED), however, it not clear whether psychological stressors affect the vasculature and tissue in a manner that could contribute to vasculogenic dysfunction. A positive correlation between hyperprolactinemia, stress has been demonstrated, interestingly, prolactin was shown decrease endothelium nitric oxide endothelium- dependent vasorelaxation different vascular beds. In this study, we aimed evaluate relaxation of corpus cavernosum male pudendal artery female mice undergoing chronic unpredictable (CUS). Objective We test hypothesis impairment caused by CUS leads impaired which may cause Methods Male C57Bl/6 were submitted 28 days The removed mounted myograph reactivity. Tissues kept physiological salt solution at 37°C, constantly aerated 95%O2/5%CO2 concentration-response curves acetylcholine (ACh; 1 nM – 30 uM) phenylephrine (PE; obtained. Using non-linear regression, obtained maximal response (Emax) potency (pEC50) ACh PE. Data are expressed as mean ± S.E.M. P<0.05 considered statistically different. Results artery, neither nor changed stress. Contraction PE higher males, compared females, there trend be further increased stressed males. On other hand, males significant induced (control: 88 4% vs stress: 75 4%, n=7 each group) well contraction 1.72 0.17% 1.07 0.15%, n= 7 group). might availability, whereas can desensitization alpha-adrenergic receptors Conclusions Our findings demonstrate impairs reactivity cavernosum, development Disclosure No